The new Spirit-NOPA®-VISIR, pumped with a Spirit® 1030-70/100 industrial femtosecond laser, is Spectra-Physics’ leading platform for three-photon (3P) imaging, enabling breakthrough applications in neuroscience. 3P excitation fluorescence microscopy allows imaging at unprecedented resolution and tissue depth. Utilizing the unique capability of this Spectra-Physics’ imaging source for 3P, very high acquisition rates are possible.